[Estradiol valerate-induced polycystic ovary syndrome: an animal model study]

Polycystic ovary syndrome [PCOS] is a complex endocrine and metabolic disorder and one of the most common causes of an ovulation among women in their reproductive age. Presence of cysts in the ovaries alteration in the blood levels of gonadotropine hormones and gaining weight are some of the main characteristics of PCOS among humans. Our goal was to investigate the possible occurrence of such conditions in animal models of PCOS. Forty five Sprague Dawely rats were divided into 3 equal groups: the treatment and sham groups were intramuscularly injected by a single dose of Estradiol Valerate [4 mg/rat, dissolved in 0.4 ml] and equal volume of olive oil, respectively, and the control group without any injection. During the 12 weeks of study, the animal's weights were measured once a week. After 8 weeks, serum levels of testosterone, estrogen, progesterone, Follicular Stimulating Hormone [FSH], Latinizing Hormone [LH] and glucose were measured. Following 12 weeks, ovaries were removed and prepared for light microscopy. Histological characteristics of ovaries were observed after hematoxylin-eosin staining. Animal weight and serum level of testosterone were significantly reduced among PCOS induced rats while progesterone, LH and glucose levels were elevated. There was no significant difference in estradiol and FSH levels among different group of animals. Many cysts and degenerating follicles were observed in the treatment group. PCOS can be experimentally produced by a single injection of Estradiol Valerate in the rat, but some of the complex aspects of PCOS are not clearly defined

[Isolation and characterization of human induced pluripotent stem cells-derived mesenchymal progenitors]

Isolating human induced pluripotent stem cells [hiPS]-derived mesenchymal progenitors as a new source of mesenchymal cells which can differentiate into different lineages like adipose and bone. After 7 days of hiPS1 culture on matrigle coated dishes, spindle like cells around colonies were removed by cell scraper. These cells that had mesenchymal like morphology was characterized after 4-6 passages. Mesenchymal cell surface markers CD73, CD90, CD105, CD29, CD44 and hematopoietic cell surface markers CD34 and CD45 were analyzed by flow cytometry and cells were differentiated to osteogenic and adipogenic lineages by defined medium. Flow cytometric analysis demonstrated that hi PS1 derived mesenchymal progenitors were%98.71 +/- 0.14 CD44+,%98.51 +/- 1.02 CD29+,%87.74 +/- 3.41 CD105+,%46.65 +/- 5.76 CD73+,%98.53 +/- 0.78 CD90+ and they did not express CD34 and CD45. These cells could be differentiated to osteogenic and adipogenic lineages. hiPS1 can make mesenchymal progenitors and these cells can be a suitable substitute for mesenchymal stem cells